Domain insertion permissibility-guided engineering of allostery in ion channels
نویسندگان
چکیده
منابع مشابه
Domain–domain interactions in ion channels
347 C o m m e n t a r y Ion channels are, with a few exceptions, multi-subunit complexes made of interacting functional domains (Hille, 2001). Combining different functionalities yields ion channels with complexity, allowing them to operate like a biological micro-machine. This general design permits the selective regulation of channel behaviors, such as the single-channel conductance or the ac...
متن کاملEngineering allostery.
Allosteric proteins have great potential in synthetic biology, but our limited understanding of the molecular underpinnings of allostery has hindered the development of designer molecules, including transcription factors with new DNA-binding or ligand-binding specificities that respond appropriately to inducers. Such allosteric proteins could function as novel switches in complex circuits, meta...
متن کاملEngineering light-gated ion channels.
Ion channels are gated by a variety of stimuli, including ligands, voltage, membrane tension, temperature, and even light. Natural gates can be altered and augmented using synthetic chemistry and molecular biology to develop channels with completely new functional properties. Light-sensitive channels are particularly attractive because optical manipulation offers a high degree of spatial and te...
متن کاملEngineering allosteric protein switches by domain insertion.
Domain insertion is proving to be an effective way to construct hybrid proteins exhibiting switch-like behavior. In this strategy, two existing domains, the first exhibiting a signal recognition function and the second containing the function to be modulated, are fused such that the recognition of the signal by the first domain is transmitted to the second domain, thereby modulating its activit...
متن کاملO 13: Ion Channels in Autoimmune Neurodegeneration
Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system characterized by widespread inflammation, focal demyelination and a variable degree of axonal and neuronal loss. Ionic conductances regulate T cell activation as well as neuronal function and thus have been found to play a crucial role in MS pathogenesis. Since present therapeutical approaches are only parti...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Communications
سال: 2019
ISSN: 2041-1723
DOI: 10.1038/s41467-018-08171-0